FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10608095
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141150.070.305120
GAGCACA219200.043.273476120
AGAGCAC287250.034.90412120
AAGAGCA430750.024.086462120
GAAGAGC484100.021.061037118-119
GGAAGAG583700.017.36793118-119
CGGAAGA695950.014.899897116-117
TCGGAAG692700.014.792571118-119
ATCGGAA821950.012.595082114-115
CACACGT41250.012.443324120
GATCGGA835750.012.41568116-117
CACGTCT41450.011.887952120
AGATCGG922900.011.350381112-113
TAGATCG209650.010.982413118-119
ACACGTC44700.010.411193120
CTAGATC53450.09.891662112-113
TTAGATC83450.09.52358110-111
AAGATCG389200.09.372458120
GAGATCG316100.08.530429120
CGTCTGA67050.08.165641120
Produced by FastQC (version 0.11.5)