FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15275788
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC192750.081.83911120
GAGCACA308500.050.053543120
AGAGCAC410400.038.068165118-119
AAGAGCA638650.024.417332120
GAAGAGC716150.021.87181120
GGAAGAG842600.018.578644118-119
CGGAAGA1007950.015.801477120
TCGGAAG1038700.015.119976120
ATCGGAA1210450.013.161732118-119
GATCGGA1242100.012.984679120
CACACGT50400.012.5249405120
CTAGATC74900.012.039995120
AGATCGG1374850.011.998345120
TAGATCG283600.010.790415116-117
CACGTCT57300.010.532451120
ACGTCTG68000.010.201309120
AAGATCG623100.010.028209118-119
ACACGTC58150.09.714563118-119
TTAGATC126050.09.018092116-117
GAGATCG447500.08.975854118-119
Produced by FastQC (version 0.11.5)