FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14323255
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163100.070.30146120
GAGCACA246650.043.468586120
AGAGCAC333150.033.586666120
AAGAGCA513250.022.532907120
GAAGAGC578150.020.744354120
GGAAGAG702500.016.852076118-119
CGGAAGA875850.014.393099120
TCGGAAG892050.014.080004120
CACGTCT72150.012.878101120
ATCGGAA1030250.012.296917118-119
GATCGGA1049500.011.886041120
CACACGT74950.011.429856120
AGATCGG1145450.010.94976116-117
TAGATCG359550.010.601612114-115
ACACGTC79400.010.457289120
CTAGATC94000.010.094939120
ACGTCTG83100.09.674484120
TTAGATC137450.09.25223112-113
CCTAGAT31150.08.885058120
CAGATCG620500.08.771343116-117
Produced by FastQC (version 0.11.5)