FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15674321
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC176850.077.621605120
GAGCACA282650.046.870266120
AGAGCAC381100.035.406616118-119
AAGAGCA581750.023.608475120
GAAGAGC656850.020.813599118-119
GGAAGAG778800.017.643913120
CGGAAGA935150.015.023612120
TCGGAAG941750.014.845587120
ATCGGAA1087850.012.973174118-119
GATCGGA1102850.012.956503120
AGATCGG1222200.011.552701118-119
CTAGATC66200.010.959299114-115
TAGATCG299150.010.776024118-119
AAGATCG351550.09.489248120
TTAGATC96100.09.223192110-111
GAGATCG513200.09.19553116-117
CAGATCG415800.08.460679118-119
AGAGATC198300.07.9728613108-109
ATAGATC160450.07.912811118-119
CCTAGAT24450.07.5644064120
Produced by FastQC (version 0.11.5)