FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16770446
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC232900.078.40075120
GAGCACA373800.049.788166120
AGAGCAC502850.038.23676120
AAGAGCA762200.024.9873118-119
GAAGAGC845600.022.907682120
GGAAGAG1058250.018.128363120
CGGAAGA1228800.015.691257118-119
TCGGAAG1236600.015.343704114-115
ATCGGAA1481350.013.584558120
GATCGGA1526350.012.686191120
AGATCGG1655150.011.977864118-119
CACGTCT66350.011.8853655120
CACACGT64700.011.634448120
TAGATCG391000.010.927738120
CTAGATC78350.010.339516120
GAGATCG726500.09.729747118-119
ACACGTC68300.09.551718120
CAGATCG673350.09.26545110-111
CGTCTGA113250.08.419265120
ACGTCTG93150.08.218284118-119
Produced by FastQC (version 0.11.5)