FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22466842
Sequences flagged as poor quality0
Sequence length53-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA4519400.014.411099120
TCATTAA1418900.09.044135120
ATCATTA1573000.08.676824120
CATTAAA1367500.08.638264120
GTATCAT1751650.08.517979120
CGTATCA1785450.08.420569120
CCGTATC1949300.08.229828120
TATCATT1775750.08.034116120
GCCGTAT2080100.07.9171014120
CGCCGTA2201300.07.7782664120
GTCGCCG2507050.07.3585153120
TGGTCGC2771300.07.3409514120
TCGCCGT2390600.07.3355045120
GGTCGCC2618850.07.2620077120
GTGGTCG2950000.06.993881120
ATTAAAA1573450.06.9089704120
TTAAAAA1569700.06.8987217120
GGTGGTC3132500.06.66494120
CGGTGGT3355800.06.4431286120
TCGGTGG3519650.06.248864120
Produced by FastQC (version 0.11.5)