FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13593916
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC171900.067.69738120
GAGCACA260400.044.534275120
AGAGCAC343750.033.883034118-119
AAGAGCA515950.022.70514118-119
GAAGAGC582950.020.321108120
GGAAGAG714450.016.986645120
TCGGAAG829750.015.430665120
CGGAAGA830600.014.676194120
GATCGGA1003600.012.8584385120
ATCGGAA986150.012.76463118-119
AGATCGG1112100.011.397798118-119
CACGTCT51850.010.922888120
TTAGATC82350.010.643555120
TAGATCG225300.010.324311116-117
CTAGATC58600.09.8947315116-117
CACACGT48400.09.890538120
AAGATCG473000.09.422091120
ACACGTC53550.09.254113118-119
GAGATCG428550.08.393365116-117
CCTAGAT21350.08.210671114-115
Produced by FastQC (version 0.11.5)