FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12517969
Sequences flagged as poor quality0
Sequence length55-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT211850.09.827845120
AGTGTAG309300.09.466668120
GAGTGTA339400.09.051393120
AGATCTC213400.08.828613120
AGAGTGT369350.08.804779120
GTAGATC315100.08.473618120
AAGAGTG415900.08.367511120
TGTAGAT344150.08.3162565120
GTGTAGA314900.08.155522118-119
GTAGGGA634200.08.070122120
ATCTCGG210350.08.043848120
GTGTAGG739950.08.0357895120
TGTAGGG678250.07.8821588120
TCTCGGT189050.07.680614120
GATCTCG217850.07.6567507120
TAGGGAA603050.07.6114144120
TCGTGTA829100.07.5046263120
GGTGGTC156800.07.3087745120
CGCTAGA10701.177801E-47.2898207120
CGTGTAG782950.07.188686118-119
Produced by FastQC (version 0.11.5)