FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12517969
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC120650.060.86069120
GAGCACA186300.042.490627120
AGAGCAC246800.032.30681120
AAGAGCA397200.021.50079120
GAAGAGC449500.018.418257120
GGAAGAG544900.015.445573118-119
TCGGAAG634600.013.567865120
CGGAAGA646900.013.546159120
ATCGGAA744100.012.906394120
GATCGGA740150.012.562266120
AGATCGG829550.011.753509120
TAGATCG203450.010.408486118-119
CTAGATC47800.010.059349116-117
TTAGATC82250.09.29138120
GAGATCG332200.09.278961118-119
AAGATCG288800.08.9529120
CAGATCG276300.08.274628120
CACACGT44550.07.8623047120
TAAGATC100500.07.636153118-119
CCTAGAT20100.07.446074110-111
Produced by FastQC (version 0.11.5)