FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25132344
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA7132050.016.58509120
CATTAAA2123700.09.901666120
TCATTAA2259200.09.496339120
TTAAAAA2093650.09.247158120
ATTAAAA2183100.09.137585120
ATCATTA2480700.08.924123120
GTATCAT2805550.08.654233120
TATCATT2738800.08.492761120
TAAAAAA2150900.08.4320135120
CGTATCA2940550.08.377295120
CCGTATC3206800.08.025993120
GCCGTAT3415750.07.5578575120
CGCCGTA3651550.07.2078176120
TCGCCGT3948050.06.641474118-119
GTCGCCG4166150.06.3578343120
GGTCGCC4288000.06.244325120
TGGTCGC4466150.06.0355453120
GTGGTCG4689250.05.724118118-119
GGTGGTC4908500.05.481849120
CGGTGGT5170200.05.2011156116-117
Produced by FastQC (version 0.11.5)