FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25132344
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC249000.075.608154120
GAGCACA385500.048.90057120
AGAGCAC483100.039.10667118-119
AAGAGCA753850.024.990156118-119
GAAGAGC816400.023.121056116-117
GGAAGAG970800.019.418304116-117
TCGGAAG1092850.017.170649114-115
CGGAAGA1133150.016.749197114-115
GATCGGA1298900.014.564505112-113
ATCGGAA1313050.014.530022112-113
CACACGT75000.013.414072120
AGATCGG1432900.013.332101110-111
CACGTCT80200.012.44151120
TAGATCG353150.012.212501120
CTAGATC110950.011.632286112-113
TTAGATC151550.010.501794120
ACACGTC81850.09.923853118-119
GAGATCG581150.09.436601110-111
AAGATCG556000.09.217838118-119
CAGATCG565000.09.020326110-111
Produced by FastQC (version 0.11.5)