FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12100059
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC146700.079.98868120
GAGCACA232850.050.330338120
AGAGCAC304450.038.361885118-119
AAGAGCA463800.025.911629120
GAAGAGC516300.022.583267120
GGAAGAG633150.018.69822120
CGGAAGA746000.015.766517114-115
TCGGAAG743750.015.718569116-117
ATCGGAA871500.013.688616112-113
CACGTCT44700.013.517843120
GATCGGA882500.013.374295114-115
CACACGT42500.012.813384120
CTAGATC45900.012.422814112-113
AGATCGG963300.012.411242110-111
TAGATCG194000.011.929743112-113
ACACGTC50800.011.894638120
ACGTCTG61100.010.622041120
CCCTAGT3700.00929512410.4203511
CGTCTGA70650.09.397402120
TTAGATC62500.09.301864116-117
Produced by FastQC (version 0.11.5)