FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12225647
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC147500.075.92631120
GAGCACA240950.047.756443120
AGAGCAC315200.036.019108118-119
AAGAGCA492600.024.026966120
GAAGAGC535850.022.24433120
GGAAGAG649900.018.335648118-119
CGGAAGA741750.015.61721118-119
TCGGAAG756200.015.587168116-117
ATCGGAA890650.013.587264120
GATCGGA906850.013.15579118-119
AGATCGG1007400.011.769782118-119
TAGATCG247200.011.686772120
CACGTCT44500.011.31789120
CTAGATC66800.010.419221112-113
CACACGT38400.010.3835125118-119
GAGATCG380450.09.67112112-113
ACACGTC45050.09.316427120
TTAGATC97250.09.171095118-119
CAGATCG354350.09.129939112-113
AAGATCG312050.09.067724116-117
Produced by FastQC (version 0.11.5)