FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12172133
Sequences flagged as poor quality0
Sequence length62-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA700350.010.109526120
GCGTCGG82200.08.759379120
GGTCGCC399650.08.722888120
GTGGTCG447750.08.563065120
TGGTCGC410850.08.12729118-119
TCGGTGG572350.08.114153120
CGCCGTA350950.08.06976120
GCCGTAT334550.07.9272976120
GGTGGTC490200.07.894967120
CGTATCA273400.07.82393118-119
GTCGCCG419300.07.7989397120
TCTCGGT639550.07.62746120
ATCTCGG690350.07.5963683120
CTCGGTG618950.07.590495120
CCGTATC319600.07.490832120
CGGTGGT548050.07.46669120
AGATCTC760550.07.281785120
GTATCAT301750.07.2578754120
TCGCCGT399950.07.141101120
ATCATTA282100.07.125341120