FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15087483
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC134250.056.337406120
GAGCACA205600.036.97225120
AGAGCAC259500.030.077919120
GAAGAGC440400.019.501112120
AAGAGCA447900.018.847645120
GGAAGAG572850.015.125574120
TCGGAAG655700.013.826094120
CGGAAGA670200.013.180238118-119
GATCGGA785450.012.004144120
ATCGGAA822000.011.540089120
AGATCGG847050.011.183778120
TAGATCG246200.010.640853118-119
CTAGATC60050.09.488417114-115
AAGATCG340450.08.358895120
TTAGATC110800.08.331494120
GAGATCG273150.08.064354118-119
CCTAGAT24400.07.9578424110-111
CACGTCT51500.07.7262273118-119
ACACGTC48900.07.6813545118-119
CAGATCG295100.07.3566465120
Produced by FastQC (version 0.11.5)