FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13997043
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC145700.070.76488120
GAGCACA229700.045.22899120
AGAGCAC316350.034.788162120
AAGAGCA483650.022.467266118-119
GAAGAGC551500.019.845592118-119
GGAAGAG671700.016.158104116-117
TCGGAAG798550.014.82396120
CGGAAGA804700.014.319687120
ATCGGAA940850.012.537408118-119
GATCGGA942450.012.363316118-119
AGATCGG1040800.011.317898116-117
CTAGATC60400.011.069089120
TAGATCG245500.010.572856120
AAGATCG454650.09.111471120
TTAGATC104050.09.027971114-115
CACACGT41050.08.451544118-119
ACACGTC48200.08.295287120
GAGATCG387850.08.160446116-117
TAAGATC174450.07.448398118-119
CAGATCG337200.07.4254894116-117
Produced by FastQC (version 0.11.5)