FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13780338
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC174350.087.005066120
GAGCACA278600.054.890682120
AGAGCAC390000.039.257523118-119
AAGAGCA605900.026.040825120
GAAGAGC675450.023.121422118-119
GGAAGAG829650.019.011192118-119
CGGAAGA967600.016.241072116-117
TCGGAAG987500.015.964976116-117
ATCGGAA1166550.013.622903114-115
GATCGGA1195500.013.404062120
CTAGATC93250.012.280202120
AGATCGG1299600.012.199625112-113
TAGATCG357650.011.519909118-119
CGATCTA20200.011.3918915
CACACGT43950.011.213658120
CCGATCT78650.010.6328784
CACGTCT45000.09.824594120
TCCGATC90050.09.7231933
TTAGATC148200.09.599965116-117
GAGATCG446150.09.551967112-113
Produced by FastQC (version 0.11.5)