FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13841522
Sequences flagged as poor quality0
Sequence length59-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTG5450.0083970448.8102713
TAGATCT409550.08.452267120
TGTAGAT564150.08.082084120
GAGTGTA578350.08.059993120
TCGGTGG319350.08.0028925120
AGTGTAG542300.07.9762726120
GTGTAGA536150.07.8327823120
AGAGTGT628750.07.6906204120
GTGGTCG255350.07.6122584120
GTAGGGA1007250.07.5822043120
AGATCTC434300.07.569986120
TGGTCGC229000.07.492641120
CGGTGGT326000.07.4531827120
TCTCGGT350400.07.310854120
AAGAGTG688800.07.176925120
GGTGGTC283550.07.1514053120
TAGGGAA959650.07.1330805120
ATCTCGG400200.07.12067120
CTCGGTG355900.07.0883427118-119
GTAGATC568350.06.9667845120
Produced by FastQC (version 0.11.5)