FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12632581
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC134450.066.38584120
GAGCACA214000.044.196056120
AGAGCAC279700.035.295063120
AAGAGCA436100.023.420782120
GAAGAGC499500.020.259834118-119
GGAAGAG617500.016.531656118-119
TCGGAAG706600.014.826928120
CGGAAGA730250.014.175733120
ATCGGAA836400.012.479402118-119
GATCGGA851450.012.428057120
AGATCGG944400.011.357968120
TAGATCG213350.010.50989118-119
CTAGATC57600.010.016862116-117
TTAGATC83600.08.705621114-115
AAGATCG387100.08.608377120
GAGATCG368250.08.234781112-113
GCTAGAT27000.07.6492395116-117
CAGATCG351900.07.639071120
GCCTAGA10600.00240621236.820612120
CCTAGAT21450.06.741117120
Produced by FastQC (version 0.11.5)