FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12847962
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC132700.070.99152120
GAGCACA208200.047.655514120
AGAGCAC285800.036.903120
AAGAGCA440850.023.522886118-119
GAAGAGC501450.020.855303118-119
GGAAGAG634550.017.021172120
TCGGAAG747300.014.607978118-119
CGGAAGA759350.014.523803120
ATCGGAA882000.012.659147120
GATCGGA889150.012.410908120
AGATCGG979250.011.333879118-119
TAGATCG243700.010.5313635118-119
CTAGATC61850.09.992155114-115
TTAGATC102100.08.952487118-119
AAGATCG400750.08.904227118-119
GAGATCG344450.08.669212116-117
CAGATCG303350.08.091076120
TAAGATC152400.07.641193116-117
CCTAGAT22850.07.5441856112-113
GCCTAGA8950.00378202187.2742105120
Produced by FastQC (version 0.11.5)