FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13631376
Sequences flagged as poor quality0
Sequence length59-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG44750.013.68238120
TAGATCT261100.010.2997265120
AGTGTAG380500.09.386784120
GATCTCG239800.09.262049120
AGATCTC255200.08.961874120
GTGTAGA384050.08.94052120
AGAGTGT459400.08.911441120
GAGTGTA420600.08.7202015120
AAGAGTG546450.08.447543120
GTAGATC381000.08.429141120
GTAGGGA795550.08.164213120
CTCGGTG215450.08.107762120
GTGTAGG921650.08.050205120
TCGGTGG207950.08.024914120
ATCTCGG239700.08.013762120
GTGGTCG144350.07.9843388120
TGTAGGG846700.07.9758596120
GGGAAAG720750.07.8788166120
TCTCGGT245000.07.864905120
TAGGGAA768250.07.852682120
Produced by FastQC (version 0.11.5)