FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13631376
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135300.066.8621120
GAGCACA232450.041.41253120
AGAGCAC300000.031.232462118-119
AAGAGCA484850.019.78246118-119
GAAGAGC546200.018.780281120
GGAAGAG650650.015.220648118-119
CGGAAGA699200.014.679967120
TCGGAAG695950.014.354626118-119
GATCGGA825150.012.876519120
ATCGGAA821100.012.814477120
AGATCGG920350.011.810622120
TAGATCG244700.010.902674118-119
CTAGATC70100.010.01888120
GAGATCG298750.09.220655118-119
TTAGATC98700.09.1394825120
AAGATCG322550.09.068454118-119
CAGATCG290750.08.788936118-119
TAAGATC123750.07.9663053120
TGAGATC144500.07.3128505120
ACACGTC36700.07.0066886118-119
Produced by FastQC (version 0.11.5)