FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11601855
Sequences flagged as poor quality0
Sequence length53-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG329500.09.489318120
GAGTGTA155850.09.203304120
TAGATCT110000.09.165777120
GTGTAGG369100.09.040312120
AGAGTGT179650.08.819203120
TCGTGTA424450.08.639076120
CGTGTAG391550.08.629266120
AGTGTAG144650.08.3393135120
TAGGGAA292000.08.179992120
GTAGGGA301100.08.171951120
AAGAGTG210050.08.085677120
GTCGTGT451550.08.040854120
CGTCGTG508500.07.8366385120
GTAGATC170250.07.5083613120
AGCGTCG571400.07.4571137120
GCGTCGT544400.07.3639536120
GAGCGTC622300.07.290791120
GTGTAGA159450.07.226523118-119
GGGAAAG331150.07.1947947120
AGAGCGT665000.07.0482626120
Produced by FastQC (version 0.11.5)