FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13157187
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC169500.085.13584120
GAGCACA266550.053.931408120
AGAGCAC352750.040.61853118-119
AAGAGCA529150.026.839958118-119
GAAGAGC598200.023.860146116-117
GGAAGAG716950.019.760061116-117
CGGAAGA834750.017.263996114-115
TCGGAAG854600.016.807789114-115
CACGTCT60650.016.485136120
CACACGT58700.015.95984120
ATCGGAA995100.014.731231112-113
GATCGGA1020900.014.162512112-113
ACACGTC68300.014.062379120
ACGTCTG73450.013.397935120
AGATCGG1118000.013.199778110-111
CGTCTGA88350.012.207696120
TAGATCG259100.012.18647112-113
CTAGATC50300.011.022522110-111
GAGATCG481750.010.64353110-111
GCACACG78700.010.503504120
Produced by FastQC (version 0.11.5)