FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15327705
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC160850.067.3479120
GAGCACA248850.044.47065120
AGAGCAC334050.032.81363118-119
AAGAGCA519600.021.46728118-119
GAAGAGC590450.019.036432120
GGAAGAG705750.016.872156120
TCGGAAG815650.014.377869120
CGGAAGA833450.014.022231118-119
ATCGGAA975800.012.161791120
GATCGGA975750.012.124244116-117
AGATCGG1081100.011.310615120
TAGATCG286500.010.802193116-117
CTAGATC68850.010.004804118-119
TTAGATC105000.09.471604120
CACGTCT53400.09.2494755120
CACACGT48500.08.945368120
CCTAGAT22400.08.765345114-115
AAGATCG410050.07.8720617116-117
GAGATCG409750.07.666869114-115
CAGATCG417400.07.4701495118-119
Produced by FastQC (version 0.11.5)