FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14417848
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166450.076.646904120
GAGCACA262400.046.89302120
AGAGCAC350250.037.36057120
AAGAGCA547650.024.199497120
GAAGAGC595100.022.18597118-119
GGAAGAG726400.018.036684118-119
CGGAAGA854850.015.656705116-117
TCGGAAG872700.015.077001116-117
ATCGGAA1032050.013.025515114-115
GATCGGA1036750.012.754783114-115
AGATCGG1137700.011.827674112-113
TAGATCG259800.011.243517120
CACACGT49700.010.520399120
CACGTCT56750.010.196229120
CTAGATC73000.09.925705114-115
GAGATCG463050.09.207487110-111
AAGATCG379600.09.048011112-113
ACACGTC60150.08.803131118-119
CAGATCG373750.08.695666112-113
TTAGATC106600.08.496603118-119
Produced by FastQC (version 0.11.5)