FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14008627
Sequences flagged as poor quality0
Sequence length53-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT142900.011.041931
GCGTCGG66100.010.891918120
CCTTCAC86700.010.3799111
GGTCGCC360900.09.991075120
TGGTCGC380400.09.857439120
CCCTAGC4950.00381710229.6963051
CCTGCAT177350.09.6074611
GTGGTCG418800.09.51231120
CATTACC106850.09.433141
CCTTTAT166600.09.1470321
GTCGCCG358650.09.100236120
CCTTGAC85900.09.0797041
GGTGGTC467000.09.031566120
GATTACC111900.08.9538111
CTCGGTG595950.08.8995285120
CCTTGAT145900.08.8410661
CCTGCAC124000.08.8058481
CCCCCAT27300.08.79060651
TCTCGGT640250.08.771035120
CCTACAT55950.08.6857321
Produced by FastQC (version 0.11.5)