FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14008627
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC193450.085.558846120
GAGCACA315550.053.49879120
AGAGCAC430450.038.9178118-119
AAGAGCA661950.025.604471118-119
GAAGAGC733150.023.993847120
GGAAGAG913300.018.99631118-119
CGGAAGA1095350.016.046959120
TCGGAAG1114700.015.869239120
ATCGGAA1334000.013.713347120
GATCGGA1320650.013.586476118-119
ACACGTC43450.013.419819120
CACACGT39250.012.886981120
AGATCGG1450350.012.359372118-119
TAGATCG288550.011.61417116-117
CACGTCT46000.010.995957120
GAGATCG620650.010.448305116-117
CCTTCAT150100.010.3915941
CATTACC105250.010.384641
CTAGATC60200.09.978963114-115
AAGATCG412100.09.9307995116-117
Produced by FastQC (version 0.11.5)