FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18337282
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC148350.063.142395120
GAGCACA231100.042.128555120
AGAGCAC309650.032.235535120
TCCGATC106250.020.8096433
AAGAGCA518400.019.97866120
CCGATCT119250.018.0518254
GAAGAGC589200.017.764616120
CGATCTA30400.017.4629885
GGAAGAG676800.015.7616005120
CTTCCGA150100.014.8446271
TCGGAAG778500.014.142982120
CGGAAGA808950.013.338727120
GATCGGA917700.012.519363120
GATCTAG9551.8189894E-1212.217166
ATCGGAA932800.011.907533120
AGATCGG1011250.011.143706120
TTCCGAT212950.010.2734762
TAGATCG250500.010.200686120
CTAGATC62600.09.610559118-119
TTAGATC110250.08.860028116-117
Produced by FastQC (version 0.11.5)