FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12913525
Sequences flagged as poor quality0
Sequence length55-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC418400.010.326325120
TCGGTGG493850.09.574941120
GTGGTCG373000.09.540053120
TGGTCGC341700.09.518298120
GGTCGCC339050.09.380307120
GCCGTAT275750.09.074558120
AGATCTC706900.09.023608120
TAGATCT747600.09.013956120
CTCGGTG546250.08.964037120
ATCTCGG621750.08.956475120
GCGTCGG93800.08.956331120
TCTCGGT566200.08.807165120
GATCTCG691250.08.767812120
CGTATCA200450.08.471988120
CGGTGGT470650.08.463968118-119
GTCGCCG371650.08.460619120
GTAGATC922000.08.343765120
AGTGTAG1005600.08.139431120
GTGTAGA961100.08.129191120
AGAGTGT1146200.07.6488256120
Produced by FastQC (version 0.11.5)