FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11737133
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC144750.075.51396120
GAGCACA234650.046.87519120
AGAGCAC314800.036.07392120
AAGAGCA475850.024.585802120
GAAGAGC539300.021.285099118-119
GGAAGAG650850.017.315393116-117
CGGAAGA762650.015.061224120
TCGGAAG764750.014.70067118-119
ATCGGAA897300.012.961077114-115
GATCGGA907950.012.673635120
AGATCGG1001400.011.593455112-113
TAGATCG221000.010.788789118-119
CTAGATC57450.010.15168114-115
CACACGT42350.09.72138120
GAGATCG307900.09.560435120
AAGATCG467550.09.42899112-113
TTAGATC96900.09.205102114-115
CACGTCT48700.08.876498120
TAAGATC179900.08.295375116-117
CAGATCG294100.08.083811116-117
Produced by FastQC (version 0.11.5)