FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13932249
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC188700.069.67045120
GAGCACA287700.046.129856120
AGAGCAC388600.034.06307118-119
AAGAGCA567050.023.594004118-119
GAAGAGC650700.020.928347120
GGAAGAG784450.017.483715120
CGGAAGA921550.015.439144120
TCGGAAG925000.015.164285118-119
CACACGT64600.014.697425120
CACGTCT63100.014.387823120
ATCGGAA1097350.013.16151120
GATCGGA1134650.012.863218120
ACACGTC74150.011.8698635120
AGATCGG1270900.011.827729120
TAGATCG261300.010.847686120
ACGTCTG80900.010.365495120
CTAGATC62200.010.139212116-117
CGTCTGA98150.09.320449120
GAGATCG522650.08.870914120
TTAGATC87400.08.8416195112-113
Produced by FastQC (version 0.11.5)