FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15517557
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG80200.09.951402120
GATCTCG554000.09.824366120
TGGTCGC282800.09.591047120
GTGGTCG312100.09.382813120
TAGATCT596550.09.264453120
GGTGGTC346600.08.933634120
TCTCGGT466550.08.913372120
TCGGTGG421450.08.785088120
AGATCTC574200.08.715853120
AGTGTAG818550.08.591904120
ATCTCGG506900.08.499809120
GTGTAGA790300.08.443448120
CGGTGGT410650.08.4170065120
GAGTGTA879150.08.340944120
GGTCGCC282450.08.306961120
CTCGGTG433050.08.286481120
GTAGATC775750.07.967507120
TGTAGAT811500.07.964054120
AGAGTGT950950.07.9067936120
GTCGCCG295700.07.6912136120
Produced by FastQC (version 0.11.5)