FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12975778
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC157950.088.6355120
GAGCACA273400.052.78337120
AGAGCAC367000.040.13137120
AAGAGCA535850.027.550117118-119
GAAGAGC613050.023.584284118-119
GGAAGAG730750.020.212269116-117
CGGAAGA847800.017.158995116-117
TCGGAAG868250.016.926208114-115
ATCGGAA1010950.014.38278114-115
GATCGGA1047950.014.073572112-113
TAGATCG283450.012.66243120
AGATCGG1149200.012.584835112-113
CAGATCG468000.010.293323110-111
GAGATCG439050.010.128705118-119
CACACGT38300.09.895046120
CTAGATC68350.09.558376116-117
TTAGATC93350.09.145838116-117
AAGATCG294700.09.127983120
ATAGATC173900.09.013102110-111
AGAGATC187050.08.819453120
Produced by FastQC (version 0.11.5)