FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14156289
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135400.064.184715120
GAGCACA206650.040.32473120
AGAGCAC267600.032.719025120
AAGAGCA445200.020.163103120
GAAGAGC471250.018.772652120
GGAAGAG567650.016.374733120
TCGGAAG655950.013.967598118-119
CGGAAGA668450.013.642928120
GATCGGA762200.012.016596116-117
ATCGGAA785900.011.54669116-117
AGATCGG847000.010.989513120
TAGATCG214050.010.628492120
CTAGATC52100.09.357123120
TTAGATC96000.08.464048116-117
CACGTCT41600.08.437607120
AAGATCG356450.08.388379120
GAGATCG289750.08.233064120
CAGATCG293800.07.654964120
CACACGT36750.07.51725118-119
ACACGTC43950.07.2469635120
Produced by FastQC (version 0.11.5)