FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11429422
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC102300.058.52676120
GAGCACA150850.041.66645120
AGAGCAC198750.030.954424120
AAGAGCA316150.019.901173118-119
GAAGAGC341000.018.041748116-117
GGAAGAG432150.015.469072120
TCGGAAG487600.014.503383120
CGGAAGA519500.013.795932120
GATCGGA583200.012.77846120
ATCGGAA587850.012.623433120
AGATCGG650600.011.444904120
TAGATCG142250.011.302795118-119
CTAGATC37800.09.899686116-117
AAGATCG300950.09.167545120
CCTAGAT13653.958121E-98.8283415120
TTAGATC63000.08.3559475120
CAGATCG247100.08.085297120
GAGATCG243800.07.830526120
TAAGATC112650.06.868926118-119
GCTAGAT18750.06.765329114-115
Produced by FastQC (version 0.11.5)