FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11251726
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC151550.093.576965120
GAGCACA255150.057.5609120
AGAGCAC344250.041.929195118-119
AAGAGCA510650.028.374367118-119
GAAGAGC580800.024.967644116-117
GGAAGAG707350.020.584429116-117
CGGAAGA809350.018.107185114-115
TCGGAAG821250.017.643011114-115
ATCGGAA962350.015.293954112-113
GATCGGA998000.014.62111112-113
AGATCGG1086900.013.566803110-111
CACACGT37800.013.361801120
TAGATCG246950.012.607006110-111
CTAGATC65100.011.637622110-111
ACACGTC43050.011.548995120
CACGTCT43200.011.326215120
AAGATCG437700.010.944253110-111
TTAGATC104450.010.615535108-109
GAGATCG356600.010.5452175112-113
CAGATCG353350.09.726542114-115
Produced by FastQC (version 0.11.5)