FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15623291
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC206750.086.61517120
GAGCACA344850.055.41337120
AGAGCAC471650.040.484962120
AAGAGCA730150.026.532501118-119
GAAGAGC788900.024.178953118-119
GGAAGAG961700.020.103088116-117
CGGAAGA1128700.017.025316118-119
TCGGAAG1169950.016.519232114-115
ATCGGAA1370150.014.081067116-117
GATCGGA1403850.013.774843112-113
AGATCGG1537000.012.498634114-115
TAGATCG329650.011.595652110-111
CTAGATC72850.010.873252112-113
AAGATCG612050.010.472352110-111
ACACGTC55700.010.459617120
CACACGT47900.010.088261118-119
GAGATCG522500.010.083568110-111
TTAGATC126500.09.937902116-117
CAGATCG455250.09.585739112-113
CACGTCT55450.09.456097120
Produced by FastQC (version 0.11.5)