FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11781654
Sequences flagged as poor quality0
Sequence length52-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1290900.013.844854120
GCGTCGG77750.011.034334120
CGTATCA525400.08.769638120
CATTAAA408850.08.716315120
TCATTAA414400.08.657489120
GTATCAT527450.08.59906120
CCGTATC599300.08.348958120
ATCATTA472350.08.255815120
CGCCGTA663500.08.119811120
GTGGTCG890950.08.033367120
TGGTCGC827700.07.958658120
GCCGTAT625800.07.861199120
GGTCGCC787700.07.8068085120
GGTGGTC949550.07.663964120
TCGCCGT735050.07.533474120
GTCGCCG775550.07.495912120
TATCATT549200.07.4719863120
TCGGTGG1094600.07.245802120
CGGTGGT1038650.07.2202344120
CTCGGTG1162400.06.905752120
Produced by FastQC (version 0.11.5)