FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11781654
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC168100.085.28087120
GAGCACA273100.054.277626120
AGAGCAC364450.039.940144118-119
AAGAGCA548100.027.447765120
GAAGAGC613250.024.022522120
GGAAGAG768400.019.349928118-119
TCGGAAG905700.016.349142116-117
CGGAAGA910250.016.209463120
CACACGT47000.014.748343120
CACGTCT47300.014.332719120
ATCGGAA1072000.013.908935118-119
ACACGTC49300.013.905778120
GATCGGA1105250.013.45236114-115
AGATCGG1198050.012.506309120
TAGATCG265250.011.730586116-117
CTAGATC49400.010.947745110-111
ACGTCTG66150.010.018186120
GAGATCG489950.010.012299120
AAGATCG373650.09.846499120
TTAGATC85300.09.822605116-117
Produced by FastQC (version 0.11.5)