FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12311881
Sequences flagged as poor quality0
Sequence length60-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT131050.011.036688120
AGTGTAG180200.010.857292120
TGTAGGG457450.09.8526945120
GTGTAGA188350.09.782086120
GAGTGTA200450.09.730519120
AGAGTGT225100.09.651434120
AAGAGTG258750.09.300853120
TAGGGAA395750.09.250551120
GTAGGGA418400.09.208779120
GTGTAGG506250.08.772541120
AGATCTC149650.08.702455120
CGTGTAG544200.08.469571120
TGTAGAT207150.08.285903120
TCGTGTA574950.08.173168120
GTCGTGT618850.08.02978120
GTAGATC214750.07.936772120
CGTCGTG717250.07.639395120
AAAGAGT305600.07.6196866120
AGCGTCG803350.07.5378084120
GAAAGAG399000.07.2649555120
Produced by FastQC (version 0.11.5)