FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12311881
Sequences flagged as poor quality0
Sequence length50-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC112950.054.317577120
GAGCACA177150.035.94019120
AGAGCAC220700.029.075157120
AAGAGCA334250.019.759758120
GAAGAGC391550.017.491644120
GGAAGAG472300.015.0710335120
TCGGAAG525350.014.3475685120
GATCGGA643300.012.923646120
CGGAAGA579250.012.839583120
ATCGGAA629400.012.473013120
AGATCGG745050.010.948624120
TAGATCG164900.010.022678120
TTAGATC58350.09.977981120
CTAGATC42400.09.154052118-119
CACACGT33350.08.259569120
CACGTCT40300.08.077908120
GAGATCG363300.07.72831118-119
AAGATCG277050.07.6374087118-119
CCTAGAT18800.07.325707116-117
CAGATCG319650.06.796031120
Produced by FastQC (version 0.11.5)