FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13287087
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC126150.072.09151120
GAGCACA202600.044.888176120
AGAGCAC274200.034.84766120
AAGAGCA422850.022.343628118-119
GAAGAGC488700.020.07037120
GGAAGAG595700.016.715948120
TCGGAAG697800.014.437566120
CGGAAGA706600.014.110597118-119
GATCGGA808800.012.7771845120
ATCGGAA809400.012.751674120
AGATCGG895650.011.733884120
TAGATCG226300.010.871165116-117
CTAGATC58150.010.270021120
TTAGATC91350.09.308848120
AAGATCG354250.09.235246118-119
GAGATCG309050.09.094784120
CAGATCG288550.08.346148120
CCTAGAT19650.07.5978913118-119
ATAGATC108050.07.5691686114-115
TAAGATC132200.07.3647294110-111
Produced by FastQC (version 0.11.5)