FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13628996
Sequences flagged as poor quality0
Sequence length84-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA188300.09.0163120
CGTGTAG248100.08.898428120
GTCGTGT277750.08.877282120
TGTAGGG215900.08.419421120
GAGCGTC401500.08.3077135120
TAGGGAA183300.08.247652120
TCGTGTA264250.08.218372120
AGCGTCG370400.08.017269120
GCGTCGT353400.07.5202003120
GATCGGA693900.07.469802120
AGAGCGT446350.07.3922973120
GTGTAGG240500.07.383612120
CGTCGTG324650.07.280699120
TCGGAAG604600.07.2335525120
GAGTGTA109800.07.10286120
TAGATCG185550.07.0483556120
AAGAGCG481350.06.954493120
CGGAAGA670450.06.92575120
CTAGATC49450.06.9151373120
ATCGGAA692550.06.9039783120
Produced by FastQC (version 0.11.5)