FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14263434
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC215650.043.763653120
GAGCACA293400.033.10267120
AGAGCAC282600.030.919395120
CACGTCT89250.019.932299120
AAGAGCA449650.019.301281118-119
GAAGAGC468350.018.628675120
ACGTCTG91850.017.801538120
ACACGTC98350.016.558264118-119
CACACGT99200.015.691162118-119
GGAAGAG567050.015.4207945120
CGTCTGA93900.015.323352120
TCGGAAG659900.014.271875120
CGGAAGA666050.014.081178120
ATCGGAA811550.012.958879120
GCACACG135850.012.469149120
TCTGAAC108100.012.160937120
GTCTGAA112850.012.054756120
GATCGGA965800.011.562808118-119
AGATCGG1070250.010.70645120
TAGATCG269250.010.323569120
Produced by FastQC (version 0.11.5)