FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17258789
Sequences flagged as poor quality0
Sequence length52-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG402550.07.841898120
GAGTGTA440950.07.79867120
TAGATCT303750.07.764823120
TGGTCGC177350.07.5800505120
GTGTAGA414800.07.3788166120
GTGGTCG201300.07.2744703120
AGAGTGT480650.07.2169585120
ATCTCGG317700.07.216081120
AGATCTC316800.07.17975120
GTAGATC401200.07.105391120
GTGTAGG827050.07.08954120
GGTGGTC221600.06.905986120
GATCTCG329500.06.9030194120
GGTCGCC194650.06.8138585120
GTAGGGA714700.06.810082120
TGTAGGG764100.06.786078120
TAGGGAA688300.06.68764120
TCTCGGT299050.06.662689120
TCGGTGG263750.06.5987363120
AAGAGTG530350.06.5105433118-119
Produced by FastQC (version 0.11.5)