FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17258789
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC147950.045.6999120
GAGCACA210950.033.005768120
AGAGCAC273950.028.078695120
AAGAGCA453350.017.286463120
GAAGAGC476200.017.262669120
GGAAGAG569950.014.74319120
TCGGAAG638700.013.0085125120
GATCGGA752950.012.546585120
CGGAAGA685700.012.281986118-119
AGATCGG824950.011.314309120
ATCGGAA788550.011.2064705120
TAGATCG199800.010.651635118-119
CTAGATC48050.010.209934120
TTAGATC85300.09.069385120
GAGATCG330800.07.904266118-119
AAGATCG335950.07.8578444118-119
GCTAGAT22451.8189894E-127.5643077120
CCCTAGA8251.3735371E-77.22908114-115
CAGATCG348950.07.101577120
CACACGT46350.06.784878120
Produced by FastQC (version 0.11.5)