FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11758152
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC142350.074.88849120
GAGCACA220850.046.37559120
AGAGCAC296350.035.47819120
AAGAGCA448150.023.46859118-119
GAAGAGC496300.021.395414120
GGAAGAG599600.018.418684120
TCGGAAG723400.015.401511120
CGGAAGA713400.015.280229118-119
ATCGGAA853750.013.22968120
GATCGGA860600.013.039313118-119
AGATCGG949600.011.622653118-119
TAGATCG233300.010.758527118-119
CTAGATC56250.010.411703112-113
CAGATCG355450.09.042478116-117
TTAGATC83800.08.902353116-117
GAGATCG370750.08.866773116-117
AAGATCG296600.08.815058116-117
CCTAGAT19850.08.781012120
GCTAGAT23450.08.324923120
CACGTCT41100.08.142626120
Produced by FastQC (version 0.11.5)