FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12024523
Sequences flagged as poor quality0
Sequence length54-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT315200.010.983297120
GCGTCGG51050.010.695197120
TGGTCGC134050.010.652542120
ATCTCGG260450.010.090039120
GTGGTCG151050.010.089186120
AGTGTAG460650.09.990045120
AGATCTC302900.09.963497120
GTGTAGA439550.09.82805120
GTAGATC428050.09.783721120
CTCGGTG232700.09.282165120
AGAGTGT546800.09.118338120
GGTCGCC134300.09.113753120
GATCTCG289100.09.110864120
GGTGGTC174200.09.092827120
TCTCGGT239250.08.95281120
GAGTGTA498700.08.866907118-119
CGGTGGT216400.08.67821120
AAGAGTG609300.08.626131120
TCGGTGG215200.08.503559120
TGTAGAT482850.08.076891120
Produced by FastQC (version 0.11.5)