FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16188108
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC148400.069.125755120
GAGCACA220800.045.236008120
AGAGCAC291900.037.37968120
GAAGAGC521400.022.135565120
AAGAGCA492650.022.041204120
GGAAGAG672650.017.225166120
TCGGAAG777000.015.2112465120
CGGAAGA801200.014.498675118-119
ATCGGAA946750.012.618654120
GATCGGA955150.012.472125118-119
AGATCGG1035350.011.406399114-115
TAGATCG266750.010.802694120
CTAGATC66200.09.408458118-119
ACACGTC45050.08.995063120
GAGATCG361750.08.650332120
AAGATCG454000.08.586532114-115
CACACGT40700.08.204722118-119
TTAGATC126500.07.999641114-115
CACGTCT49400.07.8991737120
CAGATCG357050.07.649887116-117
Produced by FastQC (version 0.11.5)